Sophie Arbefeville, MD

Assistant Professor, Department of Laboratory Medicine and Pathology

Sophie Arbefeville

Contact Info

sarbefev@umn.edu

Office Phone 612-273-2126

Mailing Address:
MMC 609 Mayo
8609 B (Campus Delivery Code)
420 Delaware St. SE
Minneapolis, MN 55455

Assistant Professor, Department of Laboratory Medicine and Pathology


Pathologist


St. George's University School of Medicine, Grenada, West Indies, 2002

San Jacinto Methodist Hospital, Bayton, TX (Family Practice), 2002-2003

State University of New York at Stony Brook (AP/CP Pathology), 2004-2008

University of Iowa, Carver College of Medicine (Medical Microbiology), 2008-2009

University of Iowa, Carver College of Medicine (Medical Microbiology Research), 2009-2010

University of Iowa, Carver College of Medicine Board Certification (Molecular Genetic Pathology), 2011-2012

Lycée Polyvalent, Mirepoix, France (Mathemathiques et Sciences de la Nature), 1986

Clinical/Anatomical Pathology Certification (#08-62): 7/31/2008-12/31/2018

Molecular Genetic Certification (#S-11-088): 9/16/2011-12/31/2021

Medical Microbiology Certification (#S-09-153): 9/15/2009-12/31/2019

Summary

Dr. Arbefeville is assistant director of the Infectious Disease Diagnostic Laboratory at University of Minnesota Medical Center and a member of the Division of Molecular Pathology and Genomics. In this capacity she conducts research in molecular assay design and development encompassing microbiology and virology pathways. In separate research she conducts ribosomal genes sequencing to identify infectious agents that cannot be identified using regular culture.

Arbefeville and her colleagues have recently developed a multiplex real-time polymerase chain reaction (PCR) assay for detecting Bordetella pertussis and Bordetella parapertussis, the infectious and highly contagious respiratory bacteria that cause whooping cough. They found their assay to be a valid method for the simultaneous detection of B. pertussis and B. parapertussis. In the transplantation arena, they have developed assays for detecting the BK virus and Epstein-Barr virus, both of which tend to infect immunosuppressed transplant patients and put them at risk. Because there are no FDA-approved tests for these viruses, major transplant centers develop their own in-house assays and sometimes share them through outreach programs.

In her ribosomal genes sequencing project Arbefeville is sequencing the 16S ribosomal RNA gene to identify bacteria and the D2 sequence domain of the 28S ribosomal RNA gene to identify fungi (yeast and mold) directly from patient specimens. Her team uses universal primers to amplify and sequence the ribosomal genes and analyze the variable regions, which are unique to each species, for information as to whether a specific bacterium or fungus is present. Direct sequencing of patient specimens represents a significant advance, in part because many infectious agents cannot be cultured using traditional cell culture techniques.

Professional Associations

  • Member, Academy of Clinical Laboratory Physicians and Scientists 2015
  • Member, American Medical Association 2015
  • Member, Association of Molecular Pathology 2011-present
  • Member, American Society for Microbiology 2006-present
  • Member, College of American Pathologists 2004-present
  • Fellow of the College of American Pathologists 2009-present
  • Member, American Society for Clinical Pathology 2004-present
  • Fellow of the American Society for Clinical Pathology 2011-present

Research

Publications

  • Barnes AMT, Crespo-Diaz RJ, Cohenour J, Kirsch JD, Arbefeville S,  Ferrieri P. A Noninvasive Rhizopus Infection With a Bladder Fungal Ball in a Patient With Poorly Controlled Diabetes Mellitus. Lab Med. 2017 Oct 23. doi: 10.1093/labmed/lmx060. [Epub ahead of print] https://www.ncbi.nlm.nih.gov/pubmed/29069422#
  • Arbefeville S, Thonen-Kerr E, Ferrieri P. Prospective and Retrospective Evaluation of the Performance of the FDA-Approved Cepheid Xpert Flu/RSV XC Assay. Lab Med. 2017 Sep 29. doi: 10.1093/labmed/lmx038. [Epub ahead of print] https://www.ncbi.nlm.nih.gov/pubmed/29036487#
  • Arbefeville S, Harris A, Ferrieri P. Comparison of sequencing the D2 region of the large subunit ribosomal RNA gene (MicroSEQ®) versus the internal transcribed spacer (ITS) regions using two public databases for identification of common and uncommon clinically relevant fungal species. J Microbiol Methods.2017Sep;140:40-46.doi:10.1016/j.mimet.2017.06.015.Epub2017Jun21. http://www.sciencedirect.com/science/article/pii/S0167701217301653
  • Sophie Arbefeville and Patricia Ferrieri. Optimizing PCR testing for the diagnosis of pertussis: current perspectives. Journal of Pathology and Laboratory International. Volume 2015:7, Page 67-71. DOI http://dx.doi.org/10.2147/PLMI.S56415
  • Sophie S. Arbefeville, Ann R. Fickle, Patricia Ferrieri. Sensitivity of the Quidel® Sofia® Fluorescent Immunoassay Compared to Two Nucleic Acid Assays and Viral Culture to Detect Pandemic Influenza A (H1N1) pdm09. Lab Med. 2015 Summer;46(3):230-4. doi: 10.1309/LMRXBN8P59ITDHQZ.
  • Shane C. McAllister, Mark R. Schleiss, Sophie Arbefeville, Marie E. Steiner, Ryan S. Hanson, Catherine Pollock, and Patricia Ferrieri. Epidemic 2014 Enterovirus D68 Cross-Reacts with Human Rhinovirus on a Respiratory Molecular Diagnostic Platform. PLoS One. 2015 Mar 23;10(3):e0118529. doi: 10.1371/journal.pone.0118529.
  • Sophie Arbefeville and Patricia Ferrieri. Comparison of Real-time PCR Positivity Rates of Bordetella pertussis Between Specimens Collected with Rayon Swab on Aluminum wire shaft in Amies Gel with Charcoal, and Flocked Swabs in Universal Viral Transport Media During an Epidemic. J Clin Microbiol. Volume 52, July 2014, Page 2656-2658.
  • Sophie Arbefeville, Michael H. Levi, Patricia Ferrieri. Development of a Multiplex Real-Time PCR Assay for the Detection of Bordetella pertussis and Bordetella parapertussis in a Single Tube Reaction. J Microbiol Methods. Volume 97, February 2014, Pages 15–19.
  • Daniel C. Marko, Ryan T. Saffert, Scott A. Cunningham, Jay Hyman, John Walsh, Sophie Arbefeville, Wanita Howard, Jon Pruessner, Nedal Safwat, Frank R. Cockerill, Aaron Bossler, Robin Patel, Sandra S. Richter. Evaluation of the Bruker Biotyper and VITEK MS Matrix-Assisted Laser Desorption Ionization-Time of Flight Mass Spectrometry Systems for the Identification of Nonfermenting Gram-Negative Bacilli From Cystic Fibrosis Patient Cultures. J Clin Microbiol. 2012 Jun; 50(6):2034-9.
  • Daniel Diekema, Sophie Arbefeville, Linda Boyken, Jennifer Kroeger, and Michael Pfaller.The Changing Epidemiology of Healthcare-Associated Candidemia over Three Decades. Diagn Microbiol Infect Dis. 2012 May; 73(1):45-8

Clinical

Specialties

  • Clinical MicrobiologyMolecular Infectious Diseases 

Board Certifications

Anatomical and Clinical Pathology