The Department of Laboratory Medicine and Pathology at the University of Minnesota Medical School is committed to bringing leading-edge basic and applied research and innovation to patient care.
The research-intensive faculty within the department have several major focus areas including cancer, immunology, cardiovascular disease, renal disease, diabetes, and genetics. The faculty consist of tenured associate and full professors, several of which currently hold endowed chairs or professorships. They interface with University of Minnesota departments and centers such as the Center for Immunology, Masonic Cancer Center, and the Institute for Translational Neuroscience.
Downtown Minneapolis viewed from a basic research laboratory
Brenner tumor (IHC for E-cadherin)
Polychromatic crystalline keratopathy of the cornea
Pap smear with endocervical adenocarcinoma in situ
Macrophages (in red) surrounding a growing ductal structure in the mammary gland
Lymph node with a micrometastasis from ductal carcinoma of the breast
Leydig cells (Reinke crystal)
Department of Laboratory Medicine and Pathology Division of Anatomic Pathology
Hematopathologist Mike Linden is visiting professor in Arequipa, Peru
EM Lab is up and running
Rachel Boney (left) and Lisa Favourite
With the recent opening of the Electron Microscopy Laboratory in the Mayo Building, LMP pathologists who want their histological diagnoses analyzed by electron microscopy can now have it done in-house. The UMMC EM Lab is equipped with a JEOL JEM 1400Plus electron microscope. The instrument is capable of magnifications ranging from 2,000x to 300,000x and of performing measurements ranging from microns to nanometers, a power essential for taking accurate measurements of basement membranes, fibrils, amyloids, and organelles. Lead tech specialist Rachel Boney said the new scope can be used to analyze any type of biological tissue for diagnostic patient care services. Currently the lab is processing renal biopsies “but we are trained to perform TEM (transmission electron microscopy) imaging on heart, liver, cilia, muscle, nerve, skin, blood, and tumors,” she said.
The JEOL instrument comes equipped with 8M pixel digital camera. In the past technicians had to process TEM images on black and white film and then develop the film in a developing room, a time-consuming process. Samples are carefully prepared by Boney and her colleague Lisa Favourite.
Flow Cytometry Conference
Flow cytometry conference features latest developments
The 2nd Annual Twin Cities Regional Flow Cytometry Conference will be held Saturday, April 1, 9:30 am. - 1:45 pm. in 450 MCRB on the Minneapolis campus.
The conference features presenters from the U of M, Mayo, Allina, and FlowJo LLC, developer of a leading analysis platform for single-cell flow and mass cytometry analysis.
Areas to be discussed include CAP flow cytometry specialty inspector training, validation of color flow cytometry instruments, surface light chain staining, B cell ontogeny, tips and tools to optimize kappa and lambda staining, and flow cytometry immunophenotyping on CSF specimens.
Registration is free and lunch will be provided. RSVP to Amy Olson: olson017 at umn.edu. RSVP needed by 3/29/16 for appropriate lunch count.
Please contact Dr. Michael Linden with any questions: linde013 at umn.edu
For more information about the conference click the link below.
Jameson receives U of M – Mayo Partnership award
Steve Jameson, LMP professor and member of the Center for Immunology, is the lead principal investigator of a research award of $996,803 through the Minnesota Partnership for Biotechnology and Medical Genomics. The grant for “Testing susceptibility of ‘dirty’ mice to induction of asthmatic disease and lung pathology” establishes a new collaboration with Hirohito Kita, a pediatric allergist and immunologist at Mayo Clinic.
The Minnesota Partnership is a collaboration between the University, the Mayo Clinic, and the state. Last year the partnership awarded seven grants totaling $6.5 million. Since it was established in 2004 it has attracted more than $60 million in grants from the National Institutes of Health.
The labs of Jameson and David Masopust (Department of Microbiology and Immunology) reported in Nature in 2016 that laboratory rodents can skew immunology research but that "dirty mice" can clean up the results. The paper, "Normalizing the environment recapitulates adult human immune traits in laboratory mice," shows that once laboratory animals are housed with pet store animals their immune systems become more similar to the human immune system.